Roads and other transportation infrastructure, such as bridges, have been shown to negatively affect wildlife populations on both small scales (e.g. vehicle-wildlife collisions) and more broadly (e.g. habitat fragmentation). However, research has shown that these effects can be mitigated through the implementation of wildlife ecopassages, which restrict wildlife access to roadways while allowing safe passage across them. To address this issue, the Lake Simcoe Conservation Authority (LSRCA) has undertaken road ecology initiatives aimed at protecting turtle populations, and has identified over 1,800 km of potential vehicle-wildlife collision (VWC) 'hotspots' within the Lake Simcoe watershed. In 2016, The LSRCA installed five wildlife 'retrofit' ecopassages within identified VWC hotspots, utilizing existing road infrastructure and wildlife exclusion fencing. Monitoring of these sites as well as paired control sites using a BACI study design has demonstrated a significant (81%) decrease in turtles killed at the ecopassage sites following construction, including an 87% decrease in adult turtle road mortality. The ecopassages were however not effective at reducing roadkill of other non-target species including snakes and amphibians. There was a peak in all wildlife killed on the roads in September, highlighting this important period for wildlife migration. Additionally, it was shown that roads with higher traffic rates had higher rates of roadkill, which could direct future road management decisions. An estimated 39% of the turtles observed at the sites used the ecopassages to cross under the roads, and 18 turtles were observed using artificial nesting structures built at the ecopassages sites. Of the eight turtles killed at the ecopassage sites post-installation, five occurred near gaps in the fencing and three occurred at the fence ends. This highlights the importance of maintaining continuous fencing to the greatest extent possible, and ensuring fence ends do not allow wildlife to pass around them. Overall, the fencing used proved to be robust and withstood all weather conditions experienced; the only maintenance issue experienced was some damage from roadside mowing. This study demonstrated that relatively inexpensive solutions (i.e. under $60,000) can be built in this and other jurisdictions using existing infrastructure to mitigate road impacts on turtle populations while maintaining habitat connectivity.