Cross-institutional partnerships and open data infrastructure offer pathways to conserve and restore habitat connectivity across jurisdictions. Often, wildlife movement data are managed using agency-specific infrastructure, and efforts to integrate data and apply them to planning decisions are slow and time consuming, limiting their impact. Existing shared data platforms and analysis software offer opportunities to streamline and harmonize data management and analysis. The Movebank platform for wildlife movement data is used by over 3,500 researchers and wildlife managers worldwide and hosts 4 billion animal location records, along with billions of other animal-borne sensor measurements, describing movements and behaviors of more than 1,200 species. Collaborations can leverage the platform’s free and flexible sharing options, live data feeds and APIs to streamline data flows. Its MoveApps analysis platform allows groups to design and share workflows to process, monitor, assess, and model data. Through Room to Roam: Y2Y Wildlife Movements—a partnership of government, non-profit, and academic partners—we are working to accelerate data analysis and coordination to improve wildlife management efforts across borders, focusing on the Yellowstone-to-Yukon migration corridor (Y2Y) of western North America. This project aims to (1) harmonize and archive partner data on Movebank; (2) identify shared needs for data management, conservation, and decision making; (3) develop shared analysis workflows on MoveApps to meet these needs; and (4) expand the partnership through communications and training opportunities with existing Movebank users and partnerships in the Y2Y. Many project partners in the region have identified road ecology and wildlife-transportation interactions as a priority for innovative data analysis. Together, we have developed public, customizable workflows to analyze wildlife interactions with road infrastructure, and to create animations of animal movements along with environmental and anthropogenic context such as road infrastructure. Together, these workflows offer flexible analysis that can integrate wildlife and road infrastructure data from multiple sources, allow professionals to efficiently explore data, and offer visualizations to engage with stakeholders. These tools are freely available to support efforts within the Y2Y and other migration corridors globally.