Roads, associated traffic, and right-of-way (ROW) fences are substantial barriers to many wildlife species including pronghorn. Traditional mitigation measures, such as overpasses for pronghorn, designed to reduce these barrier effects can be relatively expensive solutions. However, less expensive alternatives that minimize the ROW fence barrier concern and allow pronghorn to focus on navigating traffic exist, including removing the fence, setting the fence back from the road, or modifying the fence to make it more wildlife friendly, have all been implemented and tested in Arizona. In northern Arizona, an Arizona Game and Fish Department (AZGFD) a case study conducted from 2007-2011 using pronghorn telemetry data, showed that removing ROW fence increased pronghorn movements. Following that case study, AZGFD partnered with multiple stakeholders and landowners to set back and/or modify additional ROW fences along multiple northern Arizona roadways. Once completed, AZGFD conducted additional pronghorn telemetry studies (2014-2017) that indicated these efforts were only marginally successful in maintaining or increasing pronghorn movements and documented on the lower traffic volume road that previously had successful crossings during the 2007-2011, while other roads in the study had become complete barriers. Upon further analysis, AZGFD determined that between the 2007-2011 and 2014-2017 studies, traffic volumes had increased substantially on the roadways that had become complete barriers. Based on this finding, AZGFD conducted a more in-depth evaluation of pronghorn movements and traffic volumes across northern Arizona using study locations, telemetry data, and traffic volumes (n = 18) collected from 2007-2021. This evaluation determined the percentage of pronghorn that crossed roads that had standard “game fences” (16-inch or more bottom smooth wire) versus pronghorn that approached within 1 mile (1.6 km) of those same roads at various traffic volumes (844 to 33,654 AADT). The results indicated that traffic volumes directly impact the ability of pronghorn to cross roadways. At low traffic volumes (<1000 AADT), nearly 60% of collared pronghorn crossed roads but as the traffic volumes increased (>3500 AADT) less than 10% crossed, and at volumes exceeding 5,000 AADT roads became an almost complete barrier to northern Arizona pronghorn (R2 = 0.98). Although these results may vary somewhat geographically, and shift slightly higher or lower based on motivation to cross roads or fence standards, we suggest that these results provide a decision-making tool to determining when removing, setting back, or modifying ROW fences is appropriate for roads with <3500 AADT. Addressing the ROW fence issue for roadways with traffic volumes between 3500-5000 AADT may be a cost effective, short-term, “stop gap” mitigation measure to implement while long-term solutions (overpass structures) are being planned. For roadways that already have a traffic volume >5000 AADT, wildlife crossings are likely required to restore or maintain connectivity.