As human development continues to increase, so does the density of road networks and other anthropogenic structures, leading to increased impacts on the natural environment. In KwaZulu-Natal Province, the Zululand area forms a hotspot of biodiversity, with parks such as Hluhluwe-iMfolozi Park (HiP) and Isamangaliso Wetland Park (IWP) acting as large draw cards for ecotourism. As such, there is high vehicle traffic in these protected areas; additionally, HiP and IWP are bisected by major provincial roads. However, most studies have focused on the difficulties large mammals face in these parks and have neglected the smaller vertebrates in the parks. As such, this group has been severely under-represented in park management programmes that aim to reduce wildlife-vehicle collisions (WVCs). This study aimed to identify and compare the species assemblage of vertebrate WVCs, along the R618 provincial road that bisects HiP and the R22 that passes through the central section of IWP. Repeated two-hour driven surveys were conducted monthly at sunrise and sunset with identities, global positioning system (GPS) coordinates of geographical localities, photographs, measurements of distances to road shoulder and brush, road and habitat characteristics, the presence of existing traffic calming and crossing structures, and climatic conditions recorded for each carcass found. The results of this study indicate a need for increased traffic calming and crossing structures along the areas in question. The data will also be used to inform computational models to determine the ideal locations and mitigation measures that should be implemented.