Amphibians are the most vulnerable group of vertebrates. They are threatened by climate change, disease, habitat fragmentation, and habitat degradation. Habitat fragmentation due to roads negatively impacts multiple life stages of amphibians worldwide. Due to their complex natural histories, amphibians need to have habitat matrices that include both wetlands and upland habitat. In the northeastern United States, many wetlands have been separated from upland habitat due to roads. Fragmentation due to roads typically leads to extreme mortality events when amphibians cross roads during their spring migration. This mortality may lead to the extirpation of local amphibian populations. In response, conservationists have attempted to reduce road induced mortality by building amphibian underpasses. Many amphibian underpasses have been constructed across the globe. However, very few studies have compared pre- and post-construction mortality. An amphibian underpass complex was built on a road at an important amphibian crossing in Monkton, Vermont, during the summer of 2015. The underpass complex was monitored for amphibian mortality five years prior to, and five years after the construction of the underpass complex. We used mixed models and unpaired t-tests to compare the proportion of mortality before and after the construction of the underpasses. Our results showed that amphibian underpasses significantly reduced amphibian road mortality in the treatment areas. Mortality was higher for spring peepers (Pseudacris crucifer), which had the ability to climb over the crossing structure walls. However, their mortality was still lower in the treatment areas after the construction of the underpasses.