Landscape connectivity is critical to maintaining healthy wildlife populations and ecosystems. Yet, landscape and habitat connectivity are threatened around the globe. Climate change and human disturbances can restrict wildlife movements and detrimentally alter ecosystems. With increased understanding of the importance of landscape connectivity, the urgency to implement strategies to maintain connectivity grows. In the United States, significant attention has been paid to federal-level wildlife migration and connectivity policies. However, a growing number of state and local governments are also working to protect connectivity. While these state and local efforts have received less attention, they are of critical importance to maintaining connectivity and are often better aligned with governance and management authorities in wildlife, transportation, land-use, and other sectors. These localized efforts offer potential to maintain connected landscapes, yet little has been done to analyze the policies to understand different approaches at the state and local levels, including the specific sectors they target, challenges in policy implementation, and the perceived climate resiliency of the policies. Assessing state and local efforts can help advance impactful strategies to maintain connectivity and achieve synergy at scale. Building off the work of the Center for Large Landscape Conservation’s Ecological Connectivity Policy Compendium, we analyzed 36 state and 10 local connectivity policies to better understand their structure and focus. In particular, we established a framework to systematically code and characterize each policy across multiple dimensions including the mechanisms used (e.g., regulatory, procedural), policy origin (e.g., executive order, legislative action), targeted sectors, stakeholders impacted, and species of focus, among other considerations. We then used semi-structured interviews to solicit expert perspectives from key agency personnel and other stakeholders on policy implementation and impact. In this presentation, we highlight policies intersecting with the transportation sector, conceptually map the focus of these policies, and highlight gaps in implementation, coverage of species, and political durability. We conclude by identifying insights that can inform other state or local governments as they consider implementing their own landscape connectivity policies. These insights will help propel connectivity conservation and migration corridor protection forward as states and local governments consider their own policies. Ultimately, our goal is to share lessons and experiences from existing state and local policies that may be of broad relevance to other jurisdictions considering enacting their own policies to maintain ecological connectivity.