There has been very little innovation in the methods and materials used to construct wildlife crossing infrastructure since there early development in the 1970s. With the assistance of a Pooled Fund Study (PFS) this project’s goal was to design North America’s first wildlife overpass structure and associated elements using fiber-reinforced polymers (FRPs), a composite material of structural fibers set in a mold of thermoset resin. The use of FRP materials has become more prevalent over the past decade and is commonly used for pedestrian bridges and aesthetic designs in construction projects. The high strength-to-weight ratio of FRP increases the efficiency of transporting prefabricated bridge elements to the site with a construction process that utilizes smaller and more mobile equipment. This accelerated bridge construction technique, combined with a reduction in maintenance and an increased lifespan, can result in significant cost savings for using FRP compared to traditional bridge materials over their lifespan. This project evaluated manufacturers of FRP materials around the world to determine the best available commercial products to use in the final wildlife overpass design in North America. The aim was to use a real-world design sight that has plans to be mitigated for wildlife-vehicle collisions in the future with the hope that the state Department of Transportation (DOT) will continue the design to see it to completion. Site locations were proposed by the PFS Technical Advisory Committee with the final selection of the design site located along U.S. Highway 97 in Siskiyou County, California. Researchers worked with California DOT (Caltrans) project managers, engineers, and maintenance to help identify pathways and challenges to constructing FRP wildlife infrastructure along a U.S. highway. The final design incorporated an FRP girder system with a precast concrete deck using FRP rebar for the superstructure. It also used recycled plastic FRP for non-structural elements, such as fencing, sound/light barriers, jump-outs, and road access points. The cost of using FRP for wildlife infrastructure is comparable to traditional materials like concrete and steel, and can even be less expensive over the entire lifespan of the structure.