The Sky Islands region in southeast Arizona, USA, and northeast Sonora, Mexico, is highly biodiverse due to the convergence of six biotic provinces and the union of the tropics and Nearctic bioregions, making it a unique place in the world where you can find black bears living in the same habitat with jaguars, coaties and javelinas. Since 2016, we have monitored roadkill on Mexico’s Federal Highway 2, which runs east-west parallel with the USA-Mexico border, dividing the Sky Islands region in half. Our Highway 2 work has focused on the section from Ímuris to the Chihuahua state border. As of early 2022, these monitoring efforts have been extended to Federal Highway 14 in Sonora and Federal Highways 15 and 15D in Sinaloa, where at least six jaguars have been killed on the Sinaloa highways mentioned. Our goal has been to identify road mortality hotspots using roadkill data; analyze wildlife movement patterns near target stretches of highways and on bridges and culverts through camera trap research; work with expert consultants to advocate mitigation measures to include wildlife underpasses or overpasses appropriate for each hotspot; and engage local communities, federal and state transportation officials at each step of the process to gain political buy-in and support for implementation. In 2016 and 2018, 45 animal species were identified as being run over on Highway 2. Likewise, 21 species were recorded using culverts to cross safely. In 2022, including Highway 14, 15, and 15D, those numbers increased to 63 affected species, including 11 protected species such as ocelot, badger, porcupine, and river otter. In Sinaloa, ten species are using the culverts, including jaguar, ocelot, and jaguarundi. We have not recorded black bears using the culverts on Highway 2, but there have been a couple of cases of road collisions in the stretch monitored. With this data, we are providing reliable information to decision-makers and engineers at the state level on how roads affect wildlife movement and what kind of mitigation measures are needed where. In October 2019, thanks to the media efforts of Wildlands Network, the Sonora State Congress approved a bill presented by a legislator which mentions the contemplation of wildlife crossings in all new highway projects. To date, we have been providing technical advice on a new highway construction project in northeast of Sonora, which crosses a Natural Protected Area.