Transportation infrastructure, such as road networks, have contributed greatly to social and economic development in many countries, but they have been fragmenting the natural environment causing adverse effects on animal biodiversity. The purpose of the project is to examine the mandates of transportation agencies, on an international scale, that shape the way in which transport agencies view and consider biodiversity, and its integration, into their mandates. We frame the research using the concept of Ecological Economics, which promotes an interdisciplinary approach to address major environmental problems, such as biodiversity loss. We examine the following question: To what extent have transportation mandates improved over the course of the past 50 years regarding their consideration of biodiversity? We hypothesize that countries that have signed the Convention on Biological Diversity (CBD), should have biodiversity considered in their transportation mandates by now, 30 years after the original document was signed between 1992 and 1993. We use the following methods of data collection: a broad survey questionnaire to transport ministry employees, or officials, coupled with a selection of interviews, and a content analysis of transport mandates for the past 50 years (1970-2020) from key player countries including Canada, the United States, Germany, France, Switzerland, and the Netherlands. We hope to understand which ministries should be held accountable for roads having adverse effects on biodiversity. We additionally want to understand if there have been any shifts in how transport ministries view biodiversity and if their mandates reflect newer and more ‘ecologically advanced’ ways of integrating biodiversity. Research will continue until March 2023 and the results will be presented at the ICOET conference. We anticipate that results will reveal if, and by how much, the inclusion of biodiversity in transport mandates has improved and if the consideration of biodiversity has started being integrated in road planning and management plans according to the opinions of respondents. The most important implication of the research is to identify leadership models and push for better biodiversity conservation strategies at higher levels of government through policies, and underlying legislation, including the mandates of relevant ministries.