Maintaining landscape connectivity is important for the persistence of wildlife populations, preventing potentially negative genetic and demographic consequences of fragmentation. The northeastern United States is an important region for wildlife movement and connectivity, especially as the climate changes and species undergo range shifts. The state of Vermont contains multiple important wildlife linkage areas identified by groups involved in regional connectivity research. However, over 25,000 kilometers of roadway fragment the Vermont landscape and impede wildlife movement and connectivity. Terrestrial wildlife passage across road barriers can be facilitated by improving existing transportation structures, including underpasses, culverts, and bridges. In Vermont, there are over 88,000 transportation structures managed by the Vermont Agency of Transportation (VTrans), of which nearly 6,000 have been identified for their potential to facilitate wildlife movement. A collaboration between VTrans, The Nature Conservancy, Vermont Fish & Wildlife Department, and the University of Vermont implemented a project to rank these existing transportation structures by their connectivity value for eight important terrestrial mammal species. As a part of the symposium titled “System-Wide Connectivity Advancements: Using Vermont’s Terrestrial Passage Screening Tool,” we will discuss the methodology used to rank structures according to their connectivity value, including the development of omnidirectional circuit-based models of wildlife movement and how they are incorporated into the ranking tool. Transportation and wildlife managers in Vermont use the Terrestrial Passage Screening Tool to easily identify transportation structures that, if improved, would have the greatest impact on improving landscape connectivity for a terrestrial mammals in Vermont.