It is projected that by 2050 around 25 million kilometers of new highways and a third of a million kilometers of railways will be built worldwide, with 90 percent of these projects in developing countries. Latin America is at the center of much of the development of new transportation infrastructure, a trend which will pose increasing challenges for the region’s tremendous diversity of wildlife. Maintaining ecological connectivity means reducing the impacts of roads and other transportation infrastructure on the landscape and wildlife. The Latin American and Caribbean Transport Working Group (LACTWG), created under the framework of the World Commission on Natural Protected Areas (WCPA) and the Connectivity Conservation Specialist Group (CCSG) of the International Union for Conservation of Nature (IUCN), is an initiative whose mission is to alert, influence, encourage and support Latin American and Caribbean societies to develop sustainable transport infrastructures and mitigate the impacts of said infrastructures on wildlife and ecological connectivity through science and advocacy. This ambitious effort is currently led by experts from Mexico, Colombia, Argentina, Costa Rica, and Canada. LACTWG focuses on informing legislative and administrative actions by providing examples of legal provisions, policies, and regulations, measuring transportation systems' impacts, monitoring solutions' effectiveness, and prioritizing locations and needs for the future. By providing technical advice, expertise in design and engineering techniques that support effective infrastructure decisions, engaging communities to promote initiatives that are sensitive to cultural concerns, and identifying strategies that promote ecological connectivity and address the effects of climate change, LACTWG is protecting wildlife one kilometer at a time.