Roads can influence wildlife behavior and survival, and the effects can be expanded by increased traffic and noise levels, creating a road-effect zone. In addition, increased noise from urban expansion can have negative influences on wildlife, including increased fitness costs, decreased health, and population fragmentation. Wildlife crossing structures (WCS) are converted or purpose-built mitigation structures that provide wildlife safe passage, habitat connectivity, and positively impact movement, and their effectiveness can be impacted by placement, structure, and landscape attributes. Wildlife crossing structures can be negatively impacted by the road-effect zone, proximity of infrastructure, and human presence. In Cameron County, the Texas Department of Transportation constructed nine wildlife crossing structures with non-continuous exclusion fencing on Farm-to-Market (FM) 106 and five WCS and 18 wildlife guards with continuous exclusion fencing on State Highway (SH) 100, to mitigate endangered ocelot (Leopardus pardalis) road mortalities. Our objectives were to: 1) determine if traffic and noise levels influence use of WCS by target species; and 2) determine if traffic noise influences vigilant behaviors of bobcats (Lynx rufus) and coyotes (Canis latrans) at the WCS. WCS were monitored with camera trap arrays that consisted of four cameras on each side, with one camera taking videos. To monitor traffic levels, two cameras with trip sensors were deployed on FM106 and SH100. Noise levels were monitored with NSRT_mk3 sound level meters at each WCS. A two-factor analysis of variance was used to compare average noise levels between WCS and the two highways. Generalized linear models were used to examine if there were associations between WCS use with traffic levels and noise levels, and to compare the percentage of time of categorized behaviors with noise levels and vehicle presence. This study will contribute to the understanding of how traffic and noise influence the use of wildlife crossing structures by target species and behavior of wildlife in response to traffic noise.