The Wildlife Program of the British Columbia Ministry of Transportation and Infrastructure (BCMOTI) is the cornerstone of wildlife protection on highways in British Columbia, Canada. This unique program is responsible for highway-related wildlife protection initiatives for British Columbia’s wild indigenous and feral non-indigenous terrestrial species. The program provides BCMOTI a single point of contact for all wildlife issues, ranging from media inquiries to technology development. The program is instrumental in BCMOTI’s efforts to protect wildlife by raising road user awareness of wildlife, directing agency wildlife-vehicle collision reduction investments, and exploring potential solutions for wildlife-vehicle collisions. As a standalone initiative, the program draws upon the expertise of BCMOTI’s environmental, structural, electrical, geotechnical and traffic safety professionals, and external wildlife professionals and academics. Building upon the experiences of other transportation agencies, the program develops, tests and refines new concepts in wildlife protection. This approach expedites wildlife protection projects by integrating them quickly into existing infrastructure and operational practices. The program relies heavily on monitoring and data analysis for its strategic investments, ranging from conventional wildlife warning signs to advanced wildlife detection systems. Building positive relationships between BCMOTI and stakeholders, including fish and game associations, First Nations and NGO’s, is an integral part of the program. The “Kootenay Wildlife Protection Initiative” and the “Northern Wildlife Protection Initiative” are the first program initiatives designed to address unique regional wildlife, social, and cultural needs. Through Social Media platforms, including Facebook and Twitter, the program is engaging a growing public audience. Wildlife Wednesday blogs provide easily accessible information to satisfy growing public interest in wildlife protection. Program focus groups engage agency personal from all levels to develop and tailor effective wildlife awareness messaging. The program’s innovative series of “Bigger than you think!” campaigns have targeted vulnerable road users, including motorcyclists and cyclists. The “Watch for BATS under bridges” initiative was developed to raise bat awareness among agency bridge engineers and technicians to promote worker health and safety, and protect endangered bat species in British Columbia. The program’s outreach efforts support youth activities, such as the British Columbia Wildlife Federation Wildkidz camps, to raise wildlife awareness among future road users. BCMOTI’s Wildlife Program provides an example of how transportation agencies can draw upon internal technical and professional resources from a wide range of disciplines to comprehensively manage and direct wildlife protection initiatives to provide wildlife, road users and taxpayers maximum benefit.