The endangered ocelot (Leopardus pardalis) is estimated to have fewer than 80 individuals remaining in the United States, with the entire population constrained to South Texas. Within this population, 40% of known ocelot mortalities are due to vehicle collisions. To prevent additional road mortalities on state highway 100 (SH 100) in Cameron County, the Texas Department of Transportation (TxDOT) constructed 11.5 km of continuous wildlife exclusion fencing and eighteen wildlife guards (WG). To maintain landscape connectivity, TxDOT also constructed five wildlife crossing structures (WCS). Camera trap arrays at each road mitigation structure monitor actual wildlife crossing and refusal rates through WCS and at WG. This baseline data collection is important however, without supplemental research it does not effectively investigate the performance of mitigation structures. Performance determines the effectiveness of each structure (i.e. its crossing and refusal rate) and is determined for each species. Performance is influenced by factors that may be categorized into two groups: location and design. Location includes biotic and abiotic factors surrounding the structure, such as landcover type and vegetation cover, which are important when estimating ocelot occupancy. Design includes abiotic factors at the structure, such as dimensions, construction materials, or the existence of catwalks. The influence of design is often impossible to compare between WCS because the designs of these structures are so varied that the influence of specific elements cannot be isolated, as is the case within the SH100 corridor where the five crossing structures utilize four designs. Alternatively, the influence of location may be isolated by comparing wildlife use at each structure to wildlife occupancy surrounding the structure, also revealing the most effective design of those tested. A control array is used for calculating wildlife presence in the surrounding area. For SH100, a novel stratified-random control array based on matching landcover type at control sites to that at compared structures proved most effective. Comparing use at structures to wildlife occupancy in the surrounding area provides a performance differential that may be compared between structures, determining the effectiveness of each overall design. The performance of entire road ecology projects may be determined the same way. This analysis was conducted for 13 south Texas species for each mitigation structure and the road corridor as a whole; with a specific focus applied to bobcats (Lynx rufus), as they are a common surrogate for potential ocelot use. Bobcats had a positive performance at WCS and a negative performance at WG, indicating structures on the SH100 are working as intended. This trend was true for nine of the other 12 species studied, and all five WCS had positive performance differentials.