Road impact causes fragmentation of ecosystems and mortality of wildlife. There are measures to reduce this impact, in Costa Rica they are being implemented by the Ministry of Transportation (MOPT) in new road projects. In the existing routes, even though some of these are known for their high mortality of wildlife, they have not implemented measures for fauna. This is mostly due to the lack of a mechanism for MOPT and Road Management Agency. In December 2019 a mechanism was established by an effort of MOPT and CONAVI a government institutions and the VAVS group, however, our governments do not have the capacity to be able to face these investigations by themselves, that is why it is important to look for strategies that include the participation of private companies and other institutions that want to join, in this case, the Peninsula Papagayo Project, a company with a very serious environmental commitment who, in collaboration with the academy and the VAVS group managed to carry out this research. The existence of 7 Environmentally Fragile Areas and Wildlife Vulnerable to Road Impact such as jaguar, cougar, and spider monkey in the route 253 shows their environmental vulnerability. This study was carried out during the years 2019 and 2020. The methods used were bibliographic review, road surveys, monitoring with camera traps, interviews, and identification of structural connectivity. 456 wild animals were recorded on the roads, of which 388 were roadkills, in 44 km during 20 days of monitoring. In camera traps, 37 species were identified such as deer, peccary, tapir, jaguarondis, white-faced monkey, howler monkey, ocelot, and paca inhabiting the margins of the route. In addition, 7 individuals of ocelots were identified crossing the route. There were 14 points where forest fragments are crossed by the route. Only one point of natural canopy connectivity was found along the route. By overlapping the sources of information (road surveys, camera traps, structural connectivity, interviews, and historical records), the sites with the greatest probability of wildlife crossing are identified. One of the main products of this study is the proposal of the design, location and quantity of measurements for arboreal species, land mammals, amphibians, reptiles and flying species. Measures such as: adaptation of drains, overpasses, underpasses, speed bumps, adaptation of bridges, and traffic signs for the crossing of fauna for the recovery of connectivity were identified for 44 sites. This report was submitted for validation by Ministry of Environments-SINAC will request the implement of measures on Route 253 to Conservación Vial. In this way, the mechanism for the implementation of measures on existing routes will be activated and new opportunities to implement measures will be opened for other routes that are impacting wildlife in Costa Rica.