Turtles play an important role in maintaining water quality of lakes and rivers, but populations face a variety of threats to long-term persistence, including mortality on our roadways. Indeed, their behavioral and physical attributes make them particularly vulnerable to vehicle strikes, and their presence on roadways creates a public safety hazard as drivers attempt to avoid them. The Minnesota Zoo has partnered with the Minnesota Department of Transportation, along with Washington, Dakota, and Hennepin County Transportation, to test the effectiveness of barriers and warning signs in reducing small animal road mortality. The project is primarily focused on reducing turtle mortality, but we collect data on all animals encountered. In 2018, thirty-two sites in the greater Twin Cities area were selected as treatment and control sites for the study. Employing a Before-After-Control-Impact (BACI) design, we collected baseline mortality data during the first year of monitoring. In 2019, treatments including chain-link fences with wrap-around end treatments, corrugated tube barriers, and wildlife warning signs were installed at different sites. We then monitored treatment and control sites for two years. Preliminary results suggest that chain-link fence barriers are very effective at reducing road mortality of larger turtles and can help direct turtles back to the wetland or to safe passageways under the road. However, they do not prevent small turtles from entering roadways. To evaluate the utility of finer mesh hardware cloth in further reducing vehicle strikes of small turtles, existing chain-link fences were retrofitted in March, 2021, and data collection will continue through the summer. Eight-inch corrugated pipes also appear to perform well as a barrier for turtles, but further study is needed at more locations. Finally, the effectiveness of wildlife warning signs remains less clear, as preliminary data suggest significant variability among sites. We anticipate that this study will provide a framework for measures that can be implemented around the state to mitigate the mortality of small wildlife on Minnesota’s roadways, thereby promoting public safety and benefitting wildlife conservation.