Maintaining landscape connectivity is crucial for wildlife as it provides access to habitat necessary for completing their life-history needs and long-term persistence in the landscape. Notably, in areas where habitat fragmentation is more pronounced, such as in urbanized and urbanizing landscapes, conservation of habitat connectivity is important for overall biodiversity conservation. Toronto and Region Conservation Authority (TRCA) completed a two-year field study (2018-2019) that included collecting road ecology data at six sites in Peel and York Region that were identified as habitat connectivity priority areas. We documented 8778 wildlife-vehicle collisions (WVCs) with frogs and toads accounting for a disproportionately large number of WVCs representing 89% of all WVCs. Species-specific data also suggested that populations of two species at risk, Jefferson salamander (Ambystoma jeffersonianum) and common snapping turtle (Chelydra serpentina serpentina), along with 23 other fauna species of regional concern in the jurisdiction are being negatively affected through WVC-related injuries and mortality. Frog and toad WVCs were highest in the summer while snake, newt, and salamander WVCs tended to be highest in the fall. Further, turtle, bird, and mammal WVCs were often highest in the spring. There was a significant positive correlation between the number of herptile WVCs and air temperature, precipitation, and relative humidity. Altogether, using the WVC data collected in 2018 and 2019, we estimate that over 1.16 million WVCs occur in a single season (April 5-November 5) along major roads in the rural area of the TRCA jurisdiction. This number is staggering and highlights the importance of avoiding the negative effects of roads through proper planning to avoid natural features prior to construction and mitigating impacts during/post-construction if avoiding natural features is not possible.