It is projected that 25 million kilometers of new roads and one-third million kilometers of rail track will be built around the world by 2050, with 90 percent in developing countries. Latin America is the focus of much of the new transportation infrastructure. Conserving ecological connectivity means reducing the impacts of roads and other transport infrastructure on the landscape. The Transport Working Group for Latin America provides tools for reducing habitat fragmentation and easing the movement of species in some of the world’s most biologically rich ecosystems. The Transport Working Group (TWG) was formed in 2017 under the IUCN WCPA Connectivity Conservation Specialist Group. The Transport Working Group for Latin America and the Caribbean (TWG-LAC) was formed in 2019 with a membership consisting mainly of biologists and specialists from lending institutions implementing solutions that decrease impacts of roads, railways, and canals on ecological connectivity. TWG-LAC has representatives from Uruguay, Ecuador, Argentina, Brazil, Colombia, Costa Rica, México, Spain, Portugal and Canada. With a primary focus on linear transport infrastructure, the Working Group devotes its expertise toward reducing wildlife mortality and making transport systems more permeable for animal movement throughout Latin America. Members also develop and apply cutting-edge science to improve human and wildlife safety by reducing conflicts and collisions. We support the Connectivity Conservation Specialist Group by providing best practices that avoid, minimize, mitigate, or compensate for the impacts of roads, railways, and canals on ecological connectivity. Projects and activities focus on the following areas: policy, science, finance, culture, practice and resilience. We advance our mission in Latin America and the Caribbean by gathering input from across diverse disciplines and geographies; producing guidance documents, reports, and case studies for use by the transport and finance sector; working to complement other international efforts in Latin America and beyond; and offering solutions that contribute to broad global goals, as well as project-level implementation.