The Nelsonville Bypass, completed in 2013, bisects the largest remaining tract of continuous forest in southeastern Ohio, including wetlands with important amphibian migration routes. Such habitat fragmentation affects amphibian populations by increasing mortality and reducing connectivity between terrestrial and aquatic habitats. As a response to alleviate road impacts, the Ohio Department of Transportation (ODOT) installed two prefabricated amphibian ecopassages with plastic barrier fencing in 2013. We began studying the effectiveness of the amphibian barrier-ecopassage system in 2015. Between 2015-2017 we documented about 14,000 dead amphibians on the road along a 1.5 mile stretch of the new highway. Furthermore, we detected road mortality was concentrated approximately 1,200 ft south of the mitigation structure, suggesting the system was inadequately placed. Meanwhile, we detected only 12 animals that successfully traversed the length of the ecopassages. The low level of ecopassage use implies the structure was insufficient at promoting safe movement under the road. In response to our findings, ODOT decommissioned the original amphibian barrier ecopassage structure and implemented a new barrier ecopassage structure in June 2018 based on our recommendations for improvement. We have been studying the effectiveness of the new mitigation system since 2018. In the area where the new structure was installed, mortality was reduced from 62% of the total mortality down to 4% between August 2018 – July 2019. We also detected 2,509 more amphibians using the 2018 ecopassages than the 2013 ecopassages. We concluded that the new barrier ecopassage system implemented in 2018 was more effective than the 2013 system yet remains imperfect. Research efforts remain ongoing to address the deficiencies in current design recommendations, but our preliminary research provides guidance on how to make road mitigation more effective for migratory amphibians.